Thyme essential oil loaded microspheres for fish fungal infection: microstructure, in vitro dynamic release and antifungal activity
Date
2020-09-25
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Journal Title
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Publisher
Taylor & Francis Online
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1464-5246
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Abstract
Aims: Evaluate the effect of varying the droplet size of microspheres charged with thyme essen tial oil (TEO-MS) on their swelling (Sw), release rate (%RR) and in vitro antifungal activity against
Saprolegnia sp.
Methods: TEO-MS obtained by ionic gelation were characterised through SEM microscopy and
X-ray microtomography. Their Sw and RR% were evaluated at simulated fish-gastrointestinal
conditions using gravimetric and spectrophotometric techniques.
Results: For all evaluated droplet sizes (p 0.05), TEO was heterogeneously distributed inside of
the MS and TEO-MS experimented agglomeration and sphericity loss after the drying process.
Under gastric conditions, the acid pH (2.9) limited the Sw (50–100%) of TEO-MS, generating a
low RR% (14–18%). Contrary, the slightly alkaline intestinal pH (8.1) favoured the Sw ( 3.2 to
3.8 times) and therefore the RR% (42–63%).
Conclusions: TEO-MS (5–100 mg/mL) presented antifungal capacity onto Saprolegnia sp. after
the simulated fish digestion, being the small droplet size once the most effective.
Description
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Citation
Journal of Microencapsulation, Vol. 38 N° 1 (2021) p. 11-21
Keywords
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Atribución-NoComercial-CompartirIgual 3.0 Chile (CC BY-NC-SA 3.0 CL)