Thyme essential oil loaded microspheres for fish fungal infection: microstructure, in vitro dynamic release and antifungal activity
Fecha
2020-10-13
Profe guía
Perfil ORCID
Título de la revista
ISSN de la revista
Título del volumen
Editor
Taylor & Francis Online
ISBN
ISSN
1464-5246
ISSNe
Resumen
Aims: Evaluate the effect of varying the droplet size of microspheres charged with thyme essen tial oil (TEO-MS) on their swelling (Sw), release rate (%RR) and in vitro antifungal activity against Saprolegnia sp. Methods: TEO-MS obtained by ionic gelation were characterised through SEM microscopy and X-ray microtomography. Their Sw and RR% were evaluated at simulated fish-astrointestinal conditions using gravimetric and spectrophotometric techniques. Results: For all evaluated droplet sizes (p 0.05), TEO was heterogeneously distributed inside of the MS and TEO-MS experimented agglomeration and sphericity loss after the drying process. Under gastric conditions, the acid pH (2.9) limited the Sw (50–100%) of TEO-MS, generating a low RR% (14–18%). Contrary, the slightly alkaline intestinal pH (8.1) favoured the Sw ( 3.2 to 3.8 times) and therefore the RR% (42–63%). Conclusions: TEO-MS (5–100 mg/mL) presented antifungal capacity onto Saprolegnia sp. after the simulated fish digestion, being the small droplet size once the most effective.
Descripción
Lugar de Publicación
Sponsorship
Citación
Journal of Microencapsulation, Vol. 38, N° 1 (2021) p. 11-21
Palabras clave
Licencia
Atribución-NoComercial-CompartirIgual 3.0 Chile (CC BY-NC-SA 3.0 CL)