Sustainable recovery of phlorotannins from Durvillaea incurvata: integrated extraction and purification with advanced characterization

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dc.contributor.authorRivera-Tovar, Pamela Raquel
dc.contributor.authorContreras-Contreras, Gabriela
dc.contributor.authorRivas-Reyes, Paulina Isabel
dc.contributor.authorPérez-Jiménez, Jara
dc.contributor.authorMartínez-Cifuentes, Maximiliano
dc.contributor.authorPérez-Correa, José Ricardo
dc.contributor.authorMariotti-Celis, María Salomé
dc.date.accessioned2025-04-10T16:26:02Z
dc.date.available2025-04-10T16:26:02Z
dc.date.issued2025-02-21
dc.description.abstractThe rising demand for bioactive compounds from marine resources highlights the need for sustainable separation technologies. This study introduces an integrated process combining ultrasound-assisted extraction (USAE) and resin purification (RP) to isolate phlorotannins from Durvillaea incurvata, a brown seaweed with significant biomedical potential. Using a 32.5% ethanol–water solvent system for USAE followed by RP on Diaion HP-20 resin, phlorotannins were enriched 2.4-fold, with simultaneous removal of interfering compounds such as mannitol (~100%), which was demonstrated by FTIR and HPLC-IR analysis. Advanced characterization using UHPLC-QToF-MS/MS identified five novel phlorotannins with polymerization degrees of 3 to 8 phloroglucinol units in both USAE extracts and post-RP. Mass balance based on spectrophotometric measurements indicated a purification factor of ~2, confirming process effectiveness. RP streams showed distinct phlorotannin profiles, with one phlorotannin exceeding 70% relative abundance. However, MS/MS results showed significantly lower recoveries than spectrophotometric data, revealing a novel insight into RP purification. These findings highlight the critical role of comprehensive chemical characterization in optimizing sustainable phlorotannin extraction from seaweed. They propose a framework for scalable, eco-efficient technologies for achieving high-purity phlorotannin recovery. This approach facilitates the development of phlorotannin-based applications in the nutraceutical and pharmaceutical industries.
dc.identifier.citationAntioxidants, Vol.14, N°3, 250 (2025) p. 1-20
dc.identifier.doihttps://doi.org/10.3390/antiox14030250
dc.identifier.issne2076-3921
dc.identifier.orcidhttps://orcid.org/0000-0001-8064-3070
dc.identifier.orcidhttps://orcid.org/0009-0001-7376-4656
dc.identifier.orcidhttps://orcid.org/0000-0003-1357-812X
dc.identifier.urihttps://hdl.handle.net/20.500.12254/4091
dc.language.isoen
dc.publisherMDPI
dc.rightsAtribución-NoComercial-CompartirIgual 3.0 Chile (CC BY-NC-SA 3.0 CL)
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/cl/
dc.subjectUHPLC-QToF MS/MS
dc.subjectBrown seaweed
dc.subjectIntegrated extraction–purification processes
dc.subjectSpectrophotometric
dc.subjectMass balance
dc.titleSustainable recovery of phlorotannins from Durvillaea incurvata: integrated extraction and purification with advanced characterization
dc.typeArticle
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