Sustainable recovery of phlorotannins from Durvillaea incurvata: integrated extraction and purification with advanced characterization
dc.contributor.author | Rivera-Tovar, Pamela Raquel | |
dc.contributor.author | Contreras-Contreras, Gabriela | |
dc.contributor.author | Rivas-Reyes, Paulina Isabel | |
dc.contributor.author | Pérez-Jiménez, Jara | |
dc.contributor.author | Martínez-Cifuentes, Maximiliano | |
dc.contributor.author | Pérez-Correa, José Ricardo | |
dc.contributor.author | Mariotti-Celis, María Salomé | |
dc.date.accessioned | 2025-04-10T16:26:02Z | |
dc.date.available | 2025-04-10T16:26:02Z | |
dc.date.issued | 2025-02-21 | |
dc.description.abstract | The rising demand for bioactive compounds from marine resources highlights the need for sustainable separation technologies. This study introduces an integrated process combining ultrasound-assisted extraction (USAE) and resin purification (RP) to isolate phlorotannins from Durvillaea incurvata, a brown seaweed with significant biomedical potential. Using a 32.5% ethanol–water solvent system for USAE followed by RP on Diaion HP-20 resin, phlorotannins were enriched 2.4-fold, with simultaneous removal of interfering compounds such as mannitol (~100%), which was demonstrated by FTIR and HPLC-IR analysis. Advanced characterization using UHPLC-QToF-MS/MS identified five novel phlorotannins with polymerization degrees of 3 to 8 phloroglucinol units in both USAE extracts and post-RP. Mass balance based on spectrophotometric measurements indicated a purification factor of ~2, confirming process effectiveness. RP streams showed distinct phlorotannin profiles, with one phlorotannin exceeding 70% relative abundance. However, MS/MS results showed significantly lower recoveries than spectrophotometric data, revealing a novel insight into RP purification. These findings highlight the critical role of comprehensive chemical characterization in optimizing sustainable phlorotannin extraction from seaweed. They propose a framework for scalable, eco-efficient technologies for achieving high-purity phlorotannin recovery. This approach facilitates the development of phlorotannin-based applications in the nutraceutical and pharmaceutical industries. | |
dc.identifier.citation | Antioxidants, Vol.14, N°3, 250 (2025) p. 1-20 | |
dc.identifier.doi | https://doi.org/10.3390/antiox14030250 | |
dc.identifier.issne | 2076-3921 | |
dc.identifier.orcid | https://orcid.org/0000-0001-8064-3070 | |
dc.identifier.orcid | https://orcid.org/0009-0001-7376-4656 | |
dc.identifier.orcid | https://orcid.org/0000-0003-1357-812X | |
dc.identifier.uri | https://hdl.handle.net/20.500.12254/4091 | |
dc.language.iso | en | |
dc.publisher | MDPI | |
dc.rights | Atribución-NoComercial-CompartirIgual 3.0 Chile (CC BY-NC-SA 3.0 CL) | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/cl/ | |
dc.subject | UHPLC-QToF MS/MS | |
dc.subject | Brown seaweed | |
dc.subject | Integrated extraction–purification processes | |
dc.subject | Spectrophotometric | |
dc.subject | Mass balance | |
dc.title | Sustainable recovery of phlorotannins from Durvillaea incurvata: integrated extraction and purification with advanced characterization | |
dc.type | Article |
Archivos
Bloque original
1 - 1 de 1
Cargando...
- Nombre:
- Rivera Tovar 2025 Antioxidants.pdf
- Tamaño:
- 3.03 MB
- Formato:
- Adobe Portable Document Format
- Descripción:
- Texto completo
Bloque de licencias
1 - 1 de 1
No hay miniatura disponible
- Nombre:
- license.txt
- Tamaño:
- 347 B
- Formato:
- Item-specific license agreed upon to submission
- Descripción: