A single bout of resistance exercise triggers mitophagy, potentially involving the ejection of mitochondria in human skeletal muscle

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dc.contributor.authorDíaz-Castro, Francisco
dc.contributor.authorTuñón-Suárez, Mauro
dc.contributor.authorRivera, Patricia
dc.contributor.authorBotella, Javier
dc.contributor.authorCancino, Jorge
dc.contributor.authorFigueroa, Ana María
dc.contributor.authorGutiérrez, Juan
dc.contributor.authorCantin, Claudette
dc.contributor.authorDeldicque, Louise
dc.contributor.authorZbinden-Foncea, Hermann
dc.contributor.authorNielsen, Joachim
dc.contributor.authorHenríquez-Olguín, Carlos
dc.contributor.authorMorselli, Eugenia
dc.contributor.authorCastro-Sepulveda, Mauricio
dc.date.accessioned2024-11-28T18:22:11Z
dc.date.available2024-11-28T18:22:11Z
dc.date.issued2024-09-24
dc.description.abstractAim The present study aimed to investigate the effects of a single bout of resistance exercise on mitophagy in human skeletal muscle (SkM). Methods Eight healthy men were recruited to complete an acute bout of one-leg resistance exercise. SkM biopsies were obtained one hour after exercise in the resting leg (Rest-leg) and the contracting leg (Ex-leg). Mitophagy was assessed using protein-related abundance, transmission electron microscopy (TEM), and fluorescence microscopy. Results Our results show that acute resistance exercise increased pro-fission protein phosphorylation (DRP1Ser616) and decreased mitophagy markers such as PARKIN and BNIP3L/NIX protein abundance in the Ex-leg. Additionally, mitochondrial complex IV decreased in the Ex-leg when compared to the Rest-leg. In the Ex-leg, TEM and immunofluorescence images showed mitochondrial cristae abnormalities, a mitochondrial fission phenotype, and increased mitophagosome-like structures in both subsarcolemmal and intermyofibrillar mitochondria. We also observed increased mitophagosome-like structures on the subsarcolemmal cleft and mitochondria in the extracellular space of SkM in the Ex-leg. We stimulated human primary myotubes with CCCP, which mimics mitophagy induction in the Ex-leg, and found that BNIP3L/NIX protein abundance decreased independently of lysosomal degradation. Finally, in another human cohort, we found a negative association between BNIP3L/NIX protein abundance with both mitophagosome-like structures and mitochondrial cristae density in the SkM. Conclusion The findings suggest that a single bout of resistance exercise can initiate mitophagy, potentially involving mitochondrial ejection, in human skeletal muscle. BNIP3L/NIX is proposed as a sensitive marker for assessing mitophagy flux in SkM.
dc.identifier.citationActa Physiologica, Vol. 240, N°9; e1403 (2024) p. 1-15.
dc.identifier.doihttps://doi.org/10.1111/apha.14203
dc.identifier.issn1748-1708
dc.identifier.issne1748-1716
dc.identifier.orcidhttps://orcid.org/0000-0003-1644-8742
dc.identifier.orcidhttps://orcid.org/0000-0002-3085-9057
dc.identifier.orcidhttps://orcid.org/0000-0003-3620-9861
dc.identifier.orcidhttps://orcid.org/0000-0002-9643-1037
dc.identifier.orcidhttp://orcid.org/0000-0002-2270-299X
dc.identifier.urihttp://hdl.handle.net/20.500.12254/3943
dc.language.isoen
dc.publisherWiley
dc.rightsAtribución-NoComercial-CompartirIgual 3.0 Chile (CC BY-NC-SA 3.0 CL)
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/cl/
dc.subjectMitochondria cristae
dc.subjectMitochondria dynamics
dc.subjectMitophagy
dc.titleA single bout of resistance exercise triggers mitophagy, potentially involving the ejection of mitochondria in human skeletal muscle
dc.typeArticle
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