A single bout of resistance exercise triggers mitophagy, potentially involving the ejection of mitochondria in human skeletal muscle

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Acta Physiologica - 2024 - Díaz‐Castro - A single bout of resistance exercise triggers mitophagy potentially involving the.pdf (118.16 KB)
Fecha
2024-09-24
Autores
Profe guía
Perfil ORCID
https://orcid.org/0000-0003-1644-8742
 https://orcid.org/0000-0002-3085-9057
 https://orcid.org/0000-0003-3620-9861
 https://orcid.org/0000-0002-9643-1037
 http://orcid.org/0000-0002-2270-299X
Título de la revista
ISSN de la revista
Título del volumen
Editor
Wiley
ISBN
ISSN
1748-1708
ISSNe
1748-1716
DOI
https://doi.org/10.1111/apha.14203
URI
http://hdl.handle.net/20.500.12254/3943
Resumen
Aim The present study aimed to investigate the effects of a single bout of resistance exercise on mitophagy in human skeletal muscle (SkM). Methods Eight healthy men were recruited to complete an acute bout of one-leg resistance exercise. SkM biopsies were obtained one hour after exercise in the resting leg (Rest-leg) and the contracting leg (Ex-leg). Mitophagy was assessed using protein-related abundance, transmission electron microscopy (TEM), and fluorescence microscopy. Results Our results show that acute resistance exercise increased pro-fission protein phosphorylation (DRP1Ser616) and decreased mitophagy markers such as PARKIN and BNIP3L/NIX protein abundance in the Ex-leg. Additionally, mitochondrial complex IV decreased in the Ex-leg when compared to the Rest-leg. In the Ex-leg, TEM and immunofluorescence images showed mitochondrial cristae abnormalities, a mitochondrial fission phenotype, and increased mitophagosome-like structures in both subsarcolemmal and intermyofibrillar mitochondria. We also observed increased mitophagosome-like structures on the subsarcolemmal cleft and mitochondria in the extracellular space of SkM in the Ex-leg. We stimulated human primary myotubes with CCCP, which mimics mitophagy induction in the Ex-leg, and found that BNIP3L/NIX protein abundance decreased independently of lysosomal degradation. Finally, in another human cohort, we found a negative association between BNIP3L/NIX protein abundance with both mitophagosome-like structures and mitochondrial cristae density in the SkM. Conclusion The findings suggest that a single bout of resistance exercise can initiate mitophagy, potentially involving mitochondrial ejection, in human skeletal muscle. BNIP3L/NIX is proposed as a sensitive marker for assessing mitophagy flux in SkM.
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Citación
Acta Physiologica, Vol. 240, N°9; e1403 (2024) p. 1-15.
Palabras clave
Mitochondria cristae, Mitochondria dynamics, Mitophagy
Licencia
Atribución-NoComercial-CompartirIgual 3.0 Chile (CC BY-NC-SA 3.0 CL)
Licencia URL
http://creativecommons.org/licenses/by-nc-sa/3.0/cl/
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Artículos de Revistas