In house validation for the direct determination of 5-hydroxymethyl-2- furfural (HMF) in “dulce de leche''

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dc.contributor.authorBarrera, Josefinaes
dc.contributor.authorPedreschi, Francoes
dc.contributor.authorGómez, Juan Pabloes
dc.contributor.authorZúñiga, Rommy. Zes
dc.contributor.authorMariotti-Celis, María Salomées
dc.date.accessioned2021-12-13T15:39:41Z
dc.date.available2021-12-13T15:39:41Z
dc.date.issued2020-10-01
dc.description.abstractIn this work an HPLC-DAD determination method for free and total HMF in “dulce de leche” was developed and validated. The separation was carried out using a mobile phase of acetonitrile-water (4.5:95.5, v/v) on Acclaim™120 C18 (4.6 × 150 mm, 5 μm), at 30 °C and a flow rate of 1 mL/min. The detection was done at 284 nm.The method showed good selectivity and linearity (determination coefficient over 0.999). Low LOD and LOQ values were obtained (19.51 μg/L and 65.05 μg/L respectively). Relative standard deviation (RSD) for repeatability and intermediate precision was <4.12 % and <5.98 % respectively. Recovery rates were between 89.04 % and 91.89 %. In addition, the HMF contents in commercial “dulce de leche” from Chile was reported, ranging from 2.33 to 10.31 mg/kg for free HMF and 314.61–464.98 mg/kg for total HMF.en
dc.identifier.citationJournal of Food Composition and Analysis, Vol. 95, January, 103665 (2021) p. 1-5en
dc.identifier.doihttps://doi.org/10.1016/j.jfca.2020.103665
dc.identifier.issn0889-1575
dc.identifier.orcidhttps://orcid.org/0000-0003-1357-812X
dc.identifier.urihttp://hdl.handle.net/20.500.12254/2092
dc.language.isoenes
dc.publisherElsevieres
dc.rightsAtribución-NoComercial-CompartirIgual 3.0 Chile (CC BY-NC-SA 3.0 CL)en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/cl/en
dc.subject.other5-hydroxymethyl-2-furfuralen
dc.subject.otherHMFen
dc.subject.otherValidationen
dc.subject.otherHPLC-DADen
dc.subject.otherDulce de lechees
dc.subject.otherDairy producten
dc.subject.otherFood analysisen
dc.subject.otherFood compositionen
dc.titleIn house validation for the direct determination of 5-hydroxymethyl-2- furfural (HMF) in “dulce de leche''en
dc.typeArtículoes
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