A mutation in desmin makes skeletal muscle less vulnerable to acute muscle damage after eccentric loading in rats

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dc.contributor.authorLanger, Henning T.
dc.contributor.authorMossakowski, Agata A.
dc.contributor.authorAvey, Alec M.
dc.contributor.authorWohlgemuth, Ross P.
dc.contributor.authorSmith, Lucas R.
dc.contributor.authorZbinden-Foncea, Herman
dc.contributor.authorBaar, Keith
dc.date.accessioned2022-01-19T12:20:36Z
dc.date.available2022-01-19T12:20:36Z
dc.date.issued2021-09-01
dc.descriptionThe current study was funded by a generous gift from the BertinBarbe Family to HZF, and KB. The work was further supported by the National Institute of Aging through project grants (R01AG056999 and R01AG45375). AAM was supported by a postdoctoral fellowship from Deutsche Forschungsgemeinschaftes
dc.description.abstractDesminopathy is the most common intermediate filament disease in humans. The most frequent mutation causing desminopathy in patients is a R350P DES missense mutation. We have developed a rat model with an analogous mutation in R349P Des. To investigate the role of R349P Des in mechanical loading, we stimulated the sciatic nerve of wild-type littermates (WT) (n = 6) and animals carrying the mutation (MUT) (n = 6) causing a lengthening contraction of the dorsi flexor muscles. MUT animals showed signs of ongoing regeneration at baseline as indicated by a higher number of central nuclei (genotype: P < .0001). While stimulation did not impact central nuclei, we found an increased number of IgG positive fibers (membrane damage indicator) after eccentric contractions with both genotypes (stimulation: P < .01). Interestingly, WT animals displayed a more pronounced increase in IgG positive fibers with stimulation compared to MUT (interaction: P < .05). In addition to altered histology, molecular signaling on the protein level differed between WT and MUT. The membrane repair protein dysferlin decreased with eccentric loading in WT but increased in MUT (interaction: P < .05). The autophagic substrate p62 was increased in both genotypes with loading (stimulation: P < .05) but tended to be more elevated in WT (interaction: P = .05). Caspase 3 levels, a central regulator of apoptotic cell death, was increased with stimulation in both genotypes (stimulation: P < .01) but more so in WT animals (interaction: P < .0001). Overall, our data indicate that R349P Des rats have a lower susceptibility to structural muscle damage of the cytoskeleton and sarcolemma with acute eccentric loading.es
dc.identifier.citationLanger HT, Mossakowski AA, Avey AM, et al. A mutation in desmin makes skeletal muscle less vulnerable to acute muscle damage after eccentric loading in rats. FASEB J. 2021;35:e21860. https://doi.org/10.1096/fj.20210 0711RRes
dc.identifier.issn35
dc.identifier.orcid0000-0002-9643-1037es
dc.identifier.orcidhttps://orcid.org/0000-0003-1838-4279es
dc.identifier.orcidhttps://orcid.org/0000-0002-7610-4231es
dc.identifier.orcidhttps://doi.org/10.1096/fj.202100711RR
dc.identifier.urihttp://hdl.handle.net/20.500.12254/2234
dc.language.isoenes
dc.publisherWiley Open Accesses
dc.relation.ispartofserieshttps://doi.org/10.1096/fj.202100711RR;
dc.relation.ispartofseriesFASEB Journal;
dc.rightsAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject.otherExercisees
dc.subject.otherFilamentes
dc.subject.otherInjuryes
dc.subject.otherIntermediatees
dc.subject.otherMusclees
dc.subject.otherSignalinges
dc.titleA mutation in desmin makes skeletal muscle less vulnerable to acute muscle damage after eccentric loading in ratses
dc.typeArtículoes
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