Severe COVID-19 correlates with lower mitochondrial cristae density in PBMCs and greater sitting time in humans

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Physiological Reports - 2022 - Castro‐Sepulveda - Severe COVID‐19 correlates with lower mitochondrial cristae density in.pdf (1.96 MB)
Fecha
2022-05-27
Autores
Profe guía
Perfil ORCID
https://orcid.org/0000-0002-2270-299X
 https://orcid.org/0000-0002-0587-1855
 https://orcid.org/0000-0002-3085-9057
 https://orcid.org/0000-0002-5948-2281
 https://orcid.org/0000-0002-9643-1037
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Editor
Wiley; The Physiological Society and the American Physiological Society
ISBN
ISSN
2051-817X
ISSNe
DOI
https://doi.org/10.14814/phy2.15369
URI
http://hdl.handle.net/20.500.12254/2537
Resumen
An interaction between mitochondrial dynamics, physical activity levels, andCOVID-19 severity has been previously hypothesized. However, this has notbeen tested. We aimed to compare mitochondrial morphology and cristae den-sity of PBMCs between subjects with non- severe COVID- 19, subjects with se-vere COVID- 19, and healthy controls. Additionally, we compared the level ofmoderate-vigorous physical activity (MVPA) and sitting time between groups.Blood samples were taken to obtain PBMCs. Mitochondrial dynamics were as-sessed by electron microscopy images and western blot of protein that regulatemitochondrial dynamics. The International Physical Activity Questionnaire(IPAQ; short version) was used to estimate the level of MVPA and the sitting timeThe patients who develop severe COVID-19 (COVID-19++) not present altera-tions of mitochondrial size neither mitochondrial density in comparison to non-severe patients COVID- 19 (COVID- 19) and control subjects (CTRL). However,compared to CTRL, COVID- 19 and COVID-19++ groups have lower mitochon-drial cristae length, a higher proportion of abnormal mitochondrial cristae. TheCOVID-19++ group has lower number (trend) and length of mitochondrial cris-tae in comparison to COVID- 19 group. COVID- 19, but not COVID- 19++ grouphad lower Opa 1, Mfn 2 and SDHB (Complex II) proteins than CTRL group.Besides, COVID-19++ group has a higher time sitting. Our results show that lowmitochondrial cristae density, potentially due to physical inactivity, is associatedwith COVID-19 severity.
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Citación
Physiological report, Vol. 10, N°14 (2022) p. 1-8.
Palabras clave
COVID-19, Metabolism, Mitochondrial dynamics, Mitochondrial fusion, SARS-CoV-2
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