The viral nucleocapsid protein and the human RNA-binding protein Mex3A promote translation of the Andes orthohantavirus small mRNA

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The viral nucleocapsid protein and the human RNA-binding protein Mex3A promote translation of the Andes orthohantavirus small mRNA.pdf (4.28 MB)
Date
2021-09-21
Authors
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https://orcid.org/0000-0002-4047-779X
Journal Title
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Publisher
Public Library of Science
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1553-7374
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https://doi.org/10.1371/journal.ppat.1009931
URI
http://hdl.handle.net/20.500.12254/2133
Abstract
Andes orthohantavirus (ANDV) is endemic in Argentina and Chile and is the primary etiological agent of hantavirus cardiopulmonary syndrome (HCPS) in South America. ANDV is unique among other members of the Hantaviridae family of viruses because of its ability to spread from person to person. The molecular mechanisms driving ANDV protein synthesis remain poorly understood. A previous report showed that translation of the Small segment mRNA (SmRNA) of ANDV relied on both the 5’cap and the 3’untranslated region (UTR) of the SmRNA. In this new study, we further characterize the mechanism by which the 5’ and 3’end of the SmRNA interact to assure viral protein synthesis. We establish that the viral nucleocapsid protein N and the cellular protein hMex3A participate in the process. These observations indicated that both viral and cellular proteins regulate viral gene expression during ANDV infection by enabling the viral mRNA to establish a non-covalent 5’-3’end interaction.
Description
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El trabajo fue financiado por la Agencia Nacional de Investigación y Desarrollo (ANID), Gobierno de Chile a través de la Iniciativa Científica Milenio (ICM), Instituto Milenio de Inmunología e Inmunoterapia (P09/016-F; ICN09_016), Programa de Investigación Asociativa PIA-ACT1408 a MLL, y FONDECYT 11150611 a JV-O, y por el Centre National de la Recherche Scientifique (CNRS, Francia), a través del programa Laboratoire International Associé (LIA) otorgado a MLL. EC-V realizó esta investigación en cumplimiento parcial de los requisitos para un Ph.D., Doctorado en Ciencias Biológicas mención Microbiología y Genética Molecular, Microbiología, Facultad de Biología, Pontificia Universidad Católica de Chile, financiado por las becas de doctorado FONDECYT 21090539 y 24121224. Los financiadores no tuvieron ningún rol en el diseño del estudio, la recopilación y análisis de datos, la decisión de publicar o la preparación del manuscrito.
Citation
Plos Pathogens, Vol. 17, N° 9, e1009931 (2021) p. 1-35
Keywords
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Atribución-NoComercial-CompartirIgual 3.0 Chile (CC BY-NC-SA 3.0 CL)
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http://creativecommons.org/licenses/by-nc-sa/3.0/cl/
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