Low abundance of Mfn2 protein correlates with reduced mitochondria-SR juxtaposition and mitochondrial cristae density in human men skeletal muscle: Examining organelle measurements from TEM images

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dc.contributor.authorCastro-Sepulveda, Mauricioes
dc.contributor.authorFernández-Verdejo, Rodrigoes
dc.contributor.authorTuñón-Suárez, Mauroes
dc.contributor.authorMorales-Zúñiga, Jorgees
dc.contributor.authorTroncoso, Mayarlinges
dc.contributor.authorJannas-Vela, Sebastianes
dc.contributor.authorZbinden-Foncea, Hermannes
dc.date.accessioned2022-01-07T15:06:17Z
dc.date.available2022-01-07T15:06:17Z
dc.date.issued2021-03-08
dc.description.abstractThe role of mitofusin 2 (Mfn2) in the regulation of skeletal muscle (SM) mitochondria-sarcoplasmic (SR) juxtaposition, mitochondrial morphology, mitochondrial cristae density (MCD), and SM quality has not been studied in humans. In in vitro studies, whether Mfn2 increases or decreases mitochondria-SR juxtaposition remains controversial. Transmission electron microscopy (TEM) images are commonly used to measure the organelle juxtaposition, but the measurements are performed "by-hand," thus potentially leading to between-rater differences. The purposes of this study were to: (1) examine the repeatability and reproducibility of mitochondrial-SR juxtaposition measurement from TEM images of human SM between three raters with different experience and (2) compare the mitochondrial-SR juxtaposition, mitochondrial morphology, MCD (stereological-method), and SM quality (cross-sectional area [CSA] and the maximum voluntary contraction [MVC]) between subjects with high abundance (Mfn2-HA; n = 6) and low abundance (Mfn2-LA; n = 6) of Mfn2 protein. The mitochondria-SR juxtaposition had moderate repeatability and reproducibility, with the most experienced raters showing the best values. There were no differences between Mfn2-HA and Mfn2-LA groups in mitochondrial size, distance from mitochondria to SR, CSA, or MVC. Nevertheless, the Mfn2-LA group showed lower mitochondria-SR interaction, MCD, and VO2max . In conclusion, mitochondrial-SR juxtaposition measurement depends on the experience of the rater, and Mfn2 protein seems to play a role in the metabolic control of human men SM, by regulating the mitochondria-SR interaction.en
dc.description.sponsorshipThis study was funded by Research Grant awarded to MCS by Universidad Finis Terrae (Grant no. CAI 2019)en
dc.identifier.citationThe FASEB Journal, Vol. 35, e21553 (2021) p. 1-12
dc.identifier.doihttps://doi.org/10.1096/fj.202002615RR
dc.identifier.issn1530-6860
dc.identifier.issne0892-6638
dc.identifier.orcidhttps://orcid.org/0000-0002-2270-299X
dc.identifier.orcidhttps://orcid.org/0000-0002-9643-1037
dc.identifier.urihttp://hdl.handle.net/20.500.12254/2225
dc.language.isoenen
dc.publisherWiley Open Accessen
dc.rightsAtribución-NoComercial-CompartirIgual 3.0 Chile (CC BY-NC-SA 3.0 CL)en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/cl/en
dc.subject.otherMICOS complexen
dc.subject.otherMitochondria dynamicsen
dc.subject.otherOrganelle communicationen
dc.subject.otherRepeatabilityen
dc.subject.otherReproducibilityen
dc.subject.otherTransmission electron microscopyen
dc.titleLow abundance of Mfn2 protein correlates with reduced mitochondria-SR juxtaposition and mitochondrial cristae density in human men skeletal muscle: Examining organelle measurements from TEM imagesen
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