Pannexin-1 expression in tumor cells correlates with colon cancer progression and survival

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dc.contributor.authorFierro Arenas, Aaron
dc.contributor.authorLandskron, Glauben
dc.contributor.authorCamhi-Vainroj, Ilan
dc.contributor.authorBasterrechea, Benjamin
dc.contributor.authorParada Venegas, Daniela
dc.contributor.authorLobos Gonzalez, Lorena
dc.contributor.authorDubois Camacho, Karen
dc.contributor.authorAraneda, Catalina
dc.contributor.authorRomero, Camila
dc.contributor.authorDominguez, Antonia
dc.contributor.authorVasquez, Gonzalo
dc.contributor.authorLopez K, Francisco
dc.contributor.authorAlvarez, Karin
dc.contributor.authorGonzalez, Carlos M
dc.contributor.authorHager Ribeiro, Carolina
dc.contributor.authorBalboa, Elisa
dc.contributor.authorEugenin, Eliseo
dc.contributor.authorHermoso, Marcela A
dc.contributor.authorDe la Fuente, Marjorie
dc.date.accessioned2024-11-22T14:05:23Z
dc.date.available2024-11-22T14:05:23Z
dc.date.issued2024
dc.description.abstractAims: Pannexin-1 (PANX1) is a hemichannel that releases ATP upon opening, initiating inflammation, cell proliferation, and migration. However, the role of PANX1 channels in colon cancer remains poorly understood, thus constituting the focus of this study. Main methods: PANX1 mRNA expression was analyzed using multiple cancer databases. PANX1 protein expression and distribution were evaluated by immunohistochemistry on primary tumor tissue and non-tumor colonic mucosa from colon cancer patients. PANX1 inhibitors (probenecid or 10Panx) were used to assess colon cancer cell lines viability. To study the role of PANX1 in vivo, a subcutaneous xenograft model using HCT116 cells was performed in BALB/c NOD/SCID immunodeficient mice to evaluate tumor growth under PANX1 inhibition using probenecid. Key findings: PANX1 mRNA was upregulated in colon cancer tissue compared to non-tumor colonic mucosa. Elevated PANX1 mRNA expression in tumors correlated with worse disease-free survival. PANX1 protein abundance was increased on tumor cells compared to epithelial cells in paired samples, in a cancer stagedependent manner. In vitro and in vivo experiments indicated that blocking PANX1 reduced cell viability and tumor growth. Significance: PANX1 can be used as a biomarker of colon cancer progression and blocking PANX1 channel opening could be used as a potential therapeutic strategy against this disease.
dc.identifier.citationLife Sciences, Vol. 351, N° 122851 (2024) p. 1-10
dc.identifier.doihttps://doi.org/10.1016/j.lfs.2024.122851
dc.identifier.issn0024-3205
dc.identifier.issne1879-0631
dc.identifier.orcidhttps://orcid.org/0000-0002-7953-6769
dc.identifier.orcidhttps://orcid.org/0000-0003-0577-7604
dc.identifier.urihttp://hdl.handle.net/20.500.12254/3892
dc.language.isoen
dc.publisherElsevier
dc.rightsAtribución-NoComercial-CompartirIgual 3.0 Chile (CC BY-NC-SA 3.0 CL)
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/cl/
dc.subjectPANX1
dc.subjectColon cancer
dc.subjectCancer progression
dc.subjectBiomarker
dc.subjectProbenecid
dc.subjectTherapeutic targeting
dc.titlePannexin-1 expression in tumor cells correlates with colon cancer progression and survival
dc.typeArticle
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