Microencapsulated pomegranate peel extract induces mitochondrial complex IV activity and prevents mitochondrial cristae alteration in brown adipose tissue in mice fed on a high-fat diet

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dc.contributor.authorEcheverria, Francisca
dc.contributor.authorJimenez Patino, Paula
dc.contributor.authorCastro-Sepulveda, Mauricio
dc.contributor.authorBustamante, Andres
dc.contributor.authorGarcia Concha, Paula
dc.contributor.authorPoblete-Aro, Carlos
dc.contributor.authorValenzuela, Rodrigo
dc.contributor.authorGarcia-Diaz, Diego F
dc.date.accessioned2022-01-19T11:50:56Z
dc.date.available2022-01-19T11:50:56Z
dc.date.issued2021-09-28
dc.description.abstractPomegranate peel is an agro-industrial residue obtained after fruit processing with high total polyphenol (TP) content, making it an attractive by-product for its reuse. Pomegranate peel extract (PPE) and its bioactive compounds have shown positive effects on obesity models. Effects on favouring mitochondrial biogenesis and function have also been described. However, once phenolic compounds are extracted, their stability can be affected by diverse factors. Microencapsulation could improve PPE stability, allowing its incorporation into functional foods. Nevertheless, studies on the potential biological effects of PPE microparticles (MPPE) in obesity models are lacking. This study aims to evaluate the effect of MPPE on brown adipose tissue (BAT) mitochondrial structure and function and metabolic alterations related to obesity in mice fed a high-fat diet (HFD). PPE was microencapsulated by spray drying using inulin (IN) as a wall material and physically-chemically characterised. Eight-week-old male C57BL/6J mice (n 40) were randomly distributed into five groups: control diet (CD), HFD, HFD + IN, HFD + PPE (50 mg/kg per d TP) and HFD + MPPE (50 mg/kg per d TP), for 14 weeks. A glucose tolerance test and indirect calorimetry were conducted. Blood and adipose tissue samples were obtained. MPPE supplementation prevented HFD-induced body weight gain (P < 0·001), fasting glycaemia (P = 0·007) and total cholesterol rise (P = 0·001). MPPE resulted in higher BAT mitochondrial complex IV activity (P = 0·03) and prevented HFD-induced mitochondrial cristae alteration (P = 0·02). In conclusion, MPPE prevented HFD-induced excessive body weight gain and associated metabolic disturbances, potentially by activating complex IV activity and preserving mitochondrial cristae structure in BAT in mice fed with a HFD.es
dc.description.sponsorshipThis work was supported by the Departamento de Nutricion, Facultad de Medicina, Universidad de Chile. The authors thank Greys Lagos and Karla Vasquez for technical assistance, Kharen Espinosa for animal care support and Elia Vasquez for helping with mice diet preparation. The authors thank professors Luis Videla (Facultad de Medicina, Universidad de Chile) and Carolina Valenzuela (Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile) for providing chemicals/ reagents and facilitating the use of the mini spray dryer, respectively. This work was also supported by FIA (PYT-2018-0309) and FONDECYT (11169541) and by the Unidad de Microscopia Avanzada, Universidad Catolica, Chile. This work was financially supported by the Agencia Nacional de Investigacion y Desarrollo (ANID; D. F. G.-D., FONDECYT grant number 1171550 and F. E., doctoral scholarship CONICYT-PFCHA/Doctorado Nacional/2017-21170196). ANID had no role in the design, analysis or writing of this article.es
dc.identifier.citationEcheverria, F., Jimenez Patino, P., Castro-Sepulveda, M., Bustamante, A., Garcia Concha, P., Poblete-Aro, C., . . . Garcia-Diaz, D. (2021). Microencapsulated pomegranate peel extract induces mitochondrial complex IV activity and prevents mitochondrial cristae alteration in brown adipose tissue in mice fed on a high-fat diet. British Journal of Nutrition, 126(6), 825-836. doi:10.1017/S000711452000481Xes
dc.identifier.orcid0000-0002-9661-0377es
dc.identifier.orcidhttps://orcid.org/0000-0003-4843-2382es
dc.identifier.orcidhttps://orcid.org/0000-0001-5055-3795es
dc.identifier.orcidhttps://orcid.org/0000-0002-7551-0553es
dc.identifier.orcidhttps://doi.org/10.1017/S000711452000481X
dc.identifier.urihttp://hdl.handle.net/20.500.12254/2232
dc.language.isoenes
dc.publisherCambridge University Presses
dc.relation.ispartofseriesBritish Journal of Nutrition;
dc.rightsAtribución-NoComercial-CompartirIgual 3.0 Chile (CC BY-NC-SA 3.0 CL)
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/cl/
dc.subject.otherPomegranate peel extractes
dc.subject.otherMicroencapsulationes
dc.subject.otherObesityes
dc.subject.otherMitochondrial functiones
dc.subject.otherMitochondrial cristaees
dc.titleMicroencapsulated pomegranate peel extract induces mitochondrial complex IV activity and prevents mitochondrial cristae alteration in brown adipose tissue in mice fed on a high-fat dietes
dc.typeArtículoes
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