Examinando por Autor "Villalva, Marisol"

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    Ítem
    A sustainable approach to obtain polyphenols from Chilean wild murta, Ugni candollei B., and Ugni molinae T., using eutectic solvents and advanced extraction techniques
    (Elsevier, 2025-04-04) Fuentes-Jorquera, Natalia; Villalva, Marisol; Pérez-Jiménez, Jara; González-Miquel, María; González, Emilio J.; Mariotti-Celis, María Salomé; Pérez-Correa, José Ricardo; Canales, Roberto I.
    Murta, native to southern Chile, comprises red murta (Ugni molinae Turcz) and white murta (Ugni candollei Barm), traditionally utilized in ethnobotanical medicine for its anti-inflammatory and analgesic properties attributed to high flavonoid and phenolic acid content. Despite murta’s potential, the combined effects of sustainable extraction techniques—eutectic solvents (ES), microwave-assisted extraction (MAE), and ultrasoundassisted extraction (UAE)—on its polyphenol profile remain unexplored. A comprehensive analysis should quantify extractable polyphenols (EPP) and non-extractable polyphenols (NEPP). This study evaluated eight ES mixtures for polyphenol extraction from red and white murta leaves and fruits, optimizing water percentage and feed:solvent ratio. Choline chloride:1,3-butanediol (ChCl:1,3BD) with 30 % water and a 1:10 ratio yielded the highest EPP content, as determined by HPLC-DAD. Among various MAE and UAE conditions tested, MAE at 353 K for 3 min achieved optimal phenolic compound yields, with catechin predominating in leaf extracts and gallic acid in fruit extracts. NEPP fractions, consisting primarily of non-extractable proanthocyanidins, represented 8–19 % of total polyphenols in fruits and leaves. These findings establish a sustainable methodology for obtaining polyphenol-rich extracts from murta and highlight the importance of both EPP and NEPP fractions in enhancing the potential of these antioxidant-enriched food extracts obtained through eco-friendly technologies.
  • Miniatura
    Ítem
    Improved extraction of phenolic compounds from fruits, leaves, and stems of Ugni candollei B. and Ugni molinae T. using pressurized aqueous glycerol
    (Elsevier, 0011-02-26) Fuentes-Jorquera, Natalia; Villalva, Marisol; Pérez-Jiménez, Jara; Mariotti-Celis, María Salomé; Canales, Roberto I; Pérez-Correa, José Ricardo
    This study explores the polyphenolic composition and antioxidant potential of fruits, leaves, and stems from two Chilean murta species: Ugni molinae Turcz (red murta) and Ugni candollei Barm (white murta). While the fruits have been previously studied, limited information exists on the phytochemical content of leaves and, in particular, stems, representing a significant knowledge gap. To address this, polyphenols were extracted using pressurized liquid extraction (PLE) with an aqueous glycerol mixture and compared to conventional maceration with aqueous acetone. Spectrophotometric analysis showed that leaves yielded the highest extractable polyphenols (EPP; up to 131 mg GAE/g dried extract) via PLE, followed by stems and fruits. Antioxidant capacity, evaluated by DPPH and ORAC assays, was strongly correlated with EPP (Spearman coefficients: 0.870 and 0.818), with red murta leaf extracts exhibiting 90 % and 65 % higher antioxidant capacity than stems and fruits, respectively. UHPLC-ESI-MS-QTOF identified 37 phenolic compounds with organ-specific distribution: flavonols and anthocyanins in fruits, myricetin and tannins (corilagin) in leaves, and catechin and dihydroflavonols in stems. Notably, murta stems previously uncharacterized proved to be valuable sources of unique polyphenols that complement those found in fruits and leaves. PLE significantly improved the extraction of certain bioactive compounds from leaves and stems (e.g., quinic acid 71 %, gallic acid 33 %, catechin 13 %), while conventional maceration was more effective for selected fruit compounds (e.g., quercetin-3-O-glucuronide 37 %). These findings expand the phytochemical knowledge of Ugni spp. and support the valorization of underutilized plant parts as functional ingredients for food and nutraceutical applications.