Seasonal transcriptome profile across three different tissues of the Andean Killifish Orestias ascotanensis

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dc.contributor.authorMaldonado-Agurto, Rodrigo
dc.contributor.authorAcevedo, Elvis
dc.contributor.authorMaracaja-Coutinho, Vinicius
dc.contributor.authorMontecino, Martin
dc.date.accessioned2025-06-17T16:28:36Z
dc.date.available2025-06-17T16:28:36Z
dc.date.issued2025-06-10
dc.description.abstractThe Andean killifish Orestias ascotanensis inhabits the high-altitude Ascotán Salt Pan, an environment with variable salinity, high UV exposure, low oxygen, and extreme daily temperature fluctuations. These conditions make it an excellent model for studying high-altitude fish biology. However, the transcriptomic responses of O. ascotanensis to seasonal acclimation remain unexplored. To investigate seasonal and tissue-specific transcriptomic profiles, RNA-seq was performed on 42 libraries from gills, skin, and muscle tissues of 14 individuals collected in summer (n = 7) and winter (n = 7). Each library had a median of 105 million reads. Principal component analysis revealed strong tissue-specific expression, and seasonal differential expression analyses identified significant transcriptomic changes within each tissue. Additionally, a bioinformatics pipeline identified 10,365 high-confidence long non-coding RNAs (lncRNAs), predicted by at least three computational tools. Compared to protein-coding genes, lncRNAs exhibited higher tissue specificity, with a predominance of monoexonic structures and shorter exon lengths. This dataset provides the first comprehensive view of seasonal mRNA and lncRNA expression in O. ascotanensis tissues.
dc.description.sponsorshipThis work was supported by grants from ANID-FONDECYT 1211026 (MM); Millennium Institute Center for Genome Regulation ICN2021_044 (MM); Millennium Nucleus of Neuroepigenetics and Plasticity NCN2023_032 (MM); FONDECYT de postdoctorado 3190720 and FONDECYT de Iniciación 11220698 (RMA). The authors thank Dr. Miguel Allende (Universidad de Chile) for his assistance during the initial stages of this study.
dc.identifier.citationScientific Data, Vol.12, N°976 (2025) p. 1-11.
dc.identifier.doihttps://doi.org/10.1038/s41597-025-05345-6
dc.identifier.issne2052‑4463
dc.identifier.orcidhttps://orcid.org/0000-0002-8326-9800
dc.identifier.urihttps://hdl.handle.net/20.500.12254/4147
dc.language.isoen
dc.publisherSpringer Nature
dc.rightsAtribución-NoComercial-CompartirIgual 3.0 Chile (CC BY-NC-SA 3.0 CL)
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/cl/
dc.subjectPez killi andino
dc.subjectOrestias ascotanensis
dc.subjectPerfiles transcriptómicos estacionales y específicos de tejido
dc.titleSeasonal transcriptome profile across three different tissues of the Andean Killifish Orestias ascotanensis
dc.typeArticle
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